Angiotensin Protocols by Donna H. Wang

By Donna H. Wang

Michigan kingdom Univ., East Lansing. interprets wisdom into reproducible equipment than allow biomedical researchers to discover the function of angiotensin in healthiness and illness. Covers genetic manipulation of particular genes. comprises tools for useful reviews of the renin-angiotensin method. DNLM: Renin - Angiotensin System-physiology

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3. Plasmid vectors and reagents for standard recombinant DNA manipulations. 4. LoxP sites either present in a plasmid vector or cloned using oligonucleotides. The sequence of the loxP site is: ATAACTTCGTATAATGTATGCTATACGAAGTTAT. 5. Standard reagents for PCR and Southern blot analysis (31). 2. Generation of Cre Transgenic Mice 1. Cre-recombinase constructs (10,32). 2. Cell-specific promoter(s). 3. Tetracycline or ecdysone inducible promoter systems (Clontech, Palo Alto, CA and Stratagene, La Jolla, CA).

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2. , and Ichikawa, I. (1997) Angiotensin-independent mechanism for aldosterone synthesis during chronic extracellular fluid volume depletion. J. Clin. Invest. 99, 855–860. 3. , et al. (1996) Chimeric mice carrying ‘regional’ targeted deletion of the angiotensin type 1A receptor gene. J. Clin. Invest. 98, 1867–1877. 4. , et al. (1998) Murine double nullizygotes of the angiotensin type 1A and 1B receptor genes duplicate severe abnormal phenotypes of angiotensin nullizygotes. J. Clin. Invest. 101, 755–760.

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