Antiviral Methods and Protocols by Derek Kinchington, Raymond F. Schinazi

By Derek Kinchington, Raymond F. Schinazi

Royal London college of medication, united kingdom. moment writer, Raymond F. Schinazi, is at Emory Univ., Decatur, GA. deals easy and scientific researchers types to guage compounds potent opposed to acute and persistent infections. comprises entire assays, caliber checking out, and unpublished equipment. DNLM: Hepatitis, Viral, Human - drug remedy.

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Treat wells from the lowest concentration of drug to the highest, adding 100 µL of RP2 to each of the four wells. Add an additional 400 µL of RP2 (without drugs) to each well. ) Continue changing culture medium and adding test compounds each day for a total of 9 d. 5. Twenty-four hours following the final addition of compound, collect the culture medium and store in new 24-well plates. Transfer a 250-µL aliquot of each stored culture medium sample to 96-well U-bottomed culture plates (one plate can hold samples from up to four 24-well plates), and store at 4°C until dot-blotting is performed.

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And Hoofnagle, J. ), Grune & Stratton, New York, pp. 209–224. 3. World Health Organization (1990) WHO reports decry of neglect of world health problems. ASM News 56, 358,359. 4. , and Mason, W. S. (1991) Viral DNA synthesis, in Current Topics in Microbiology and Immunology, vol. 168. Springer-Verlag, Berlin, pp. 41–66. 5. Wang, G. H. and Seeger, C. (1993) Novel mechanism for reverse transcription in hepatitis B viruses. J. Virol. 67, 6507–6512. 6. Hirsch, R. , Lavine, J. , Varmus, H. , and Ganem, D.

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