By Arvid B. Maunsbach, Björn A. Afzelius
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PROCEEDINGS OF THE ROYAL MICROSCOPICAL SOCIETY (1999)
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Extra resources for Biomedical Electron Microscopy. Illustrated Methods and Interpretations
The tissue was embedded in Epon, and the sections were stained with uranyl acetate and lead citrate. • 115,000. 5% potassium ferrocyanide. The tissue was embedded in Epon, and the sections were stained with uranyl acetate and lead citrate. • 50,000. Potassium ferrocyanide added to the osmium tetroxide probably forms a stable complex (Karnovsky, 1971). This method renders certain cellular components, notably membranes, microtubules, and filaments, more electron dense than after postfixation in only osmium tetroxide, and the triple-layered appearance of the cytoplasmic membranes becomes accentuated (Fig.
9, 283-291. Palade, G. (1975). Intracellular aspects of the process of protein synthesis. Science 189, 347-358. , and Sommers, S. C. ) (1986-1989) "Tumor Diagnosis by Electron Microscopy," Vols. 1-3. , New York. Roth, T. , and Porter, K. R. (1964). Yolk protein uptake in the oocyte of the mosquito Aedes aegypti. L. J. Cell Biol. 20, 313-332. Sj6strand, F. S. (1990). " Academic Press, San Diego. 11 See p. 22 for text. FIXATIVES 1. Osmium Tetroxide and Glutaraldehyde at Low Magnification 2. Osmium Tetroxide and Glutaraldehyde at High Magnification 3.
Ruthenium red, when used in electron microscopy, can be regarded either as fixative or as a stain. When used for connective tissue, it stains the collagen fibers and reveals an otherwise invisible network of fibrils and proteoglycans in the extracellular matrix. Ruthenium red has also been used widely to demonstrate glycocalyx and other surface-associated carbohydrate components. , 1989). 2. FIXATIVES 45 46 8. 2. 8A Part of a proximal tubule cell from a rat kidney during endocytosis of low molecular weight dextran (Christensen and Maunsbach, 1979).