Biomedical Electron Microscopy. Illustrated Methods and by Arvid B. Maunsbach, Björn A. Afzelius

By Arvid B. Maunsbach, Björn A. Afzelius

  • ''The publication incorporates a wealth of knowledge at the interpretation of electron micrographs - in a level of element that sometimes is understood in simple terms at the paintings ground itself... This publication has safeguarded very important facets of electron microscopy for destiny morphologists.''
    --TRENDS IN mobile BIOLOGY

    ''This is a wonderful ebook that are meant to be within the arms of any new or skilled practitioner of electron microscopy. The writing is apparent and direct and the micrographs set the top commonplace of process, caliber, and usability. it is going to set a brand new average of excellence in reinforcing the significance of the cautious and applicable use of electron microscopy in glossy telephone biology. No university, college, or lifestyles technology library might be with out it.''
    --DOODY'S PUBLISHING stories

    ''It needs to be the main finished selection of artifacts to be published!...The energy of the ebook is within the transparent and succinct presentation of strategies; the massive variety of micrographs illustrating the consequences; the various examples of artifacts and different defects; the prime quality info given approximately specimen guidance for every instance; and the references on the finish of every chapter.''

    ''This beautifully illustrated assortment would definitely obtain very heavy use...''

    ''The actual price of this e-book is that it offers a recognized and top of the range reference usual opposed to which one's personal paintings should be judged and significantly evaluated. it truly is of profit to all those that paintings within the box of organic transmission electron microscopy, in addition to the clinical box indicated within the title...''

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Extra resources for Biomedical Electron Microscopy. Illustrated Methods and Interpretations

Sample text

The tissue was embedded in Epon, and the sections were stained with uranyl acetate and lead citrate. • 115,000. 5% potassium ferrocyanide. The tissue was embedded in Epon, and the sections were stained with uranyl acetate and lead citrate. • 50,000. Potassium ferrocyanide added to the osmium tetroxide probably forms a stable complex (Karnovsky, 1971). This method renders certain cellular components, notably membranes, microtubules, and filaments, more electron dense than after postfixation in only osmium tetroxide, and the triple-layered appearance of the cytoplasmic membranes becomes accentuated (Fig.

9, 283-291. Palade, G. (1975). Intracellular aspects of the process of protein synthesis. Science 189, 347-358. , and Sommers, S. C. ) (1986-1989) "Tumor Diagnosis by Electron Microscopy," Vols. 1-3. , New York. Roth, T. , and Porter, K. R. (1964). Yolk protein uptake in the oocyte of the mosquito Aedes aegypti. L. J. Cell Biol. 20, 313-332. Sj6strand, F. S. (1990). " Academic Press, San Diego. 11 See p. 22 for text. FIXATIVES 1. Osmium Tetroxide and Glutaraldehyde at Low Magnification 2. Osmium Tetroxide and Glutaraldehyde at High Magnification 3.

Ruthenium red, when used in electron microscopy, can be regarded either as fixative or as a stain. When used for connective tissue, it stains the collagen fibers and reveals an otherwise invisible network of fibrils and proteoglycans in the extracellular matrix. Ruthenium red has also been used widely to demonstrate glycocalyx and other surface-associated carbohydrate components. , 1989). 2. FIXATIVES 45 46 8. 2. 8A Part of a proximal tubule cell from a rat kidney during endocytosis of low molecular weight dextran (Christensen and Maunsbach, 1979).

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