By Manu Larcenet
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EPR signals of wheat four extruded with varying levels of cysteine added as a radical scavenger. Adaptedfrom(3) with permission. Copyright American Assoc. of Cereal Chemists, 1996. 5% SDS without the need for P-MCE to reduce S-S bonds (Figure 7). Increased low mol wt material and decreased polymeric material in SDS-PAGE analyses under reducing and non-reducing conditions showed that the solubilization resulted from radical quenching and prevention of crosslinking by radical recombination (3) (Figure 8).
Water reduces the EPR cavity Q, which is a measure of microwave energy actually delivered to the sample (3-5). ch002 31 Figure 10. Diagram of rectangular EPR cavity with standing wave patterns from separated electrical (YZ) and magnetic (XY) components of the microwave energy. Standard EPR tubes (dotted lines) protrude into the electricalfield;this interference is eliminated by using a flat cell or capillary tube (solid line). Adapted with permissionfrom(3), copyright Wiley-Interscience, 1972. as shown in Figure 10.
EPR signal of l,4-bis-(5-acetylamino-5-carboxy-l-pentyl)pyrazinium radical cation detected in a heated mixture ofN -acetyl-L-lysine and glucose a The proposed radical cation structure was further confirmed by means of LC-MS measurements. Besides the base peak at m/z 189 (100 %), corresponding to unreacted N -acetyl-L-lysine, an ion with m/z 424 (35%) was detected by LCMS of the reacted glucose/N -acetyl-L-lysine mixture (unpublished results). Because the latter ion was expected for the proposed pyrazinium radical cation, it was further characterized by LC-MS/MS.